![]() With it you can also perform large-scale analyses, visualizing and comparing PCR data collected from multiple runs with ease. ![]() The LightCycler 480 Real-Time PCR System software is capable of different analyses of absolute or relative gene quantification, end point or melting curve based genotyping, and product characterization with SYBR Green I assays or Tm calling. Combining the filters allows for multiplexing and multicolor capabilities. Independent analysis of sample positions or differences in reaction volume are made possible by a special arrangement of optical components. It also utilizes highlysensitve opticals, with a specially developed signal collection system, excitation filters ranging from 450nm to 670m, and a high-intensity xenon lamp. The LightCycler 480 Real-Time PCR System from Roche Diagnostic’s thermoblocks have improved heat-transfer technology, increasing inter-cycle reproducibility with exceptional well-to-well temperature homogeneity. ![]() The Roche LightCycler 480 Real-Time qPCR System meets a wide variety of applications such as array validation, SNP analysis, haplotyping, gene-knockdown studies, mutation scanning, and detection of nucleic acids. Run times for qPCR are under 40 minutes for a 96-well plate and under an hour for the 384-well. Reaction volumes can range from 10-100ul for 96-well formats and 5-20ul for 384-well formats. Plates also feature a cut-away corner for error free positioning. This compact benchtop instrument uses a fully integrated multi well plate that can be switched out for either 96- or 384-well plate formats without recalibration. Manufacturer: Roche Model: LightCycler 480 Real Time Thermocycler 384-Well Block P/N: 05015154001 S (more) /N: 20168 (Year 2006) Description: The Roche LightCycler 480 Real-Time PCR System offers real-time PCR analysis of gene expression, genetic variation and melting curve-based mutation. Roche LightCycler 480 qPCR PCR Real Time Thermocycler Cycler 384 -S Well Block It also has a fast cooling and heating speed that can be programmed to perform ramps. The TC-3000G is a small gradient thermal cycler and has all the features of the TC-3000 and TC-3000X but with the added feature of being able to hold half a 96 well plate in a horizontal format.Thanks to the gradient function, it can be used to optimize both annealing temperatures and MgCl2 and primer concentrations. Programmable ramp rate (heat or cold) variable in steps of 0.1✬/sec. Number of possible programs 80 with a maximum of 25 steps and password protection 35. Programming: Program naming using alphabetic keys. Final sample cooling and prior to execution. Gradient range from 20☌ to 80☌ with interval from 1☌ to 15☌. Temperature range from 4☌ to 99☌ with a (more) n accuracy of 0.1☌ and a uniformity of ☐.2☌ at 50☌.
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